Journal: Open Life Sciences

Article Title: Sini Decoction Intervention on Atherosclerosis via PPARγ-LXRα-ABCA1 Pathway in Rabbits

doi: 10.1515/biol-2018-0053

Figure Lengend Snippet: Expression profiling of PPARγ-LXRα signaling to confirm the mechanism of treating AS by SND. The PPARγ (A-D) and LXRα (E-H) proteins levels in liver, peritoneal macrophages, PMC, and adipose tissue were measured by western blotting. The relative quantities were analyzed by ImageJ software. Data shown as mean ± SEM (n = 6). # P<0.05, compared with Chow group; *P<0.05, compared with HFD group.

Article Snippet: Primary antibodies to ABCA1 and Apo-B were purchased from Abcam (MA, USA); PPARγ antibody and LXRα antibody were purchased from Proteintech (IL, USA); and ApoA-I antibody was purchased from MyBioSource (CA, USA).

Techniques: Expressing, Western Blot, Software

Journal: Drug Design, Development and Therapy

Article Title: Asiatic acid protests against myocardial ischemia/reperfusion injury via modulation of glycometabolism in rat cardiomyocyte

doi: 10.2147/DDDT.S175116

Figure Lengend Snippet: Asiatic acid increases PPARγ levels and promotes GLUT4 translocation to plasma membrane in ischemic rats. Notes: The myocardium was isolated from vehicle-, asiatic acid (AA)-, and asiatic acid plus LY294002 (AA+LY)-treated rats under normal condition and following 1 hour of myocardial ischemia and 24 hours of reperfusion, respectively. The mRNA levels of ( A ) PPARγ and ( B ) GLUT4 were determined by real-time PCR. ( C ) PPARγ, GLUT4 from plasma membrane (PM GLUT4), GLUT4, and GAPDH levels were determined by Western blot. ( D ) Quantitative analysis of PPARγ levels were normalized to GAPDH levels, and ( E ) GLUT4 translocation levels (PM GLUT4) were normalized to total GLUT4. # P <0.05, ## P <0.01 vs the sham group; ** P <0.01 vs the vehicle group; && P <0.01 vs the asiatic acid-treated group. Abbreviation: MI/R, myocardial ischemia/reperfusion.

Article Snippet: The anti-Akt and anti-phosphor-Akt antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); the anti-GSK-3β and anti-phospho-GSK-3β (Ser9) antibodies were from Cell Signaling Technology, Inc. (Danvers, MA, USA); the anti-GLUT4 and anti-PPARγ antibody was from Boster Biological Technology, Ltd. (Wuhan, China); and the anti-glyceraldehyde phosphate dehydrogenase (GAPDH) antibody was obtained from KangChen Bio-tech Inc. (Shanghai, China).

Techniques: Translocation Assay, Clinical Proteomics, Membrane, Isolation, Real-time Polymerase Chain Reaction, Western Blot

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: Sex hormone binding globulin (SHBG) modulates mitochondrial dynamics in PPARγ-depleted equine adipose derived stromal cells

doi: 10.1007/s00109-024-02459-z

Figure Lengend Snippet: The antibodies used for Western Blot

Article Snippet: PPARG , Aviva Systems Biology, ARP32880 , 1:500.

Techniques: Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Estrogen Impairs Adipose Tissue Expansion and Cardiometabolic Profile in Obese-Diabetic Female Rats

doi: 10.3390/ijms222413573

Figure Lengend Snippet: Ovariectomy and E2 treatment effects on adiponectin and mediators of adiponectin signaling pathway in female ZDF rats. ( A ) Serum adiponectin levels. WAT protein levels of ( B ) adiponectin, ( C ) ADIPOR1 and ADIPOR2, ( D ) APPL1, ( E ) PPARG. ADIPOR, adiponectin receptor protein; APPL1, adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1; PPARG, peroxisome proliferator activated receptor gamma. Protein bands were normalized to loading control GAPDH protein intensity and expressed in arbitrary units (au) related to LEAN group. Values are expressed as the mean ± SEM. Differences between obese-diabetic (SHAM) and control (LEAN) animals were analyzed by Student’s t -test. Differences between obese-diabetic groups with different hormonal manipulation were analyzed by One-way ANOVA with Fisher’s LSD post-hoc test.

Article Snippet: Antibody for adiponectin (26 kDa, 3553) was supplied by ProSci (Poway, CA, USA); antibodies for ADIPOR1 (43 kDa, sc-99183), ADIPOR2 (50 kDa, sc-46755), GAPDH (37 kDa, sc-365062), GLUT4 (53 kDa, sc-7938) and PPARG (53 kDa, sc-7196) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA); antibodies for AKT (60 kDa, cs-2920), pAKT (60 kDa, cs-4060) and APPL1 (82 kDa, cs-3858) were supplied by Cell Signaling (Danvers, MA, USA).

Techniques: